SCSB Report
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چکیده
ollaborations with the MXL utilizing Small Angle X-ray Scattering (SAXS) have yielded new insight about the proteins being studied. While crystallization results in a static picture of a single conformation of your protein, and NMR is generally restricted to a single molecule of limited size, in solution your molecule may adopt one, two, or several conformations. Cryo-EM offers one method of imaging samples in solution, Small Angle X-ray Scattering (SAXS) provides another complementary method. In the last several years analysis tools for SAXS data have grown to include tools for querying ensembles of structures to find those that best match the solution scattering profile. Some programs that do this are: MES (Hammel), EOM (Svergun), and McSAS (Pauw). While EOM and MES require a model of your sample, and an idea of its flexibility, McSAS (MonteCarlo-SAS) is an ab initio method which determines the equivalent distribution of spheres (DOS) that matches the scattering from your sample. This can be very helpful in those cases where the structure or assembly is unknown, as in the case of protein fibril formation. In the case of subtle differences, small changes in scattering properties can also be very revealing. Hura et al. Recently promoted the use of correlation coefficients in analyzing SAXS data from multiple samples. In a similar analysis we utilized the cross-X value to determine that the effect of a known activating point mutation on the shape of our target protein was identical to that of the activating ligand. C
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A Behavioral and Electrophysiological Assessment of Activity States Defined by Using the Static Charge Sensitive Bed (SCSB) Method
There is an obvious need in some fields of sleep research for objective methods which are less expensive and complicated than the standard procedure for defining sleep stages (1) or than the modern polysomnography (PSG) in which even more physiological parameters are used in addition to the EEG, EOG, and EMG recordings. The static charge sensitive bed (SCSB) is a movement sensor, also capable o...
متن کاملRespiratory challenge induces high frequency spiking on the static charge sensitive bed (SCSB).
The static charge sensitive bed (SCSB) is a simple and noninvasive device used for the detection of sleep apnoea. In addition to episodes of apnoea or hypopnoea, heavy snorers commonly present with episodes of high frequency spiking on the SCSB. These spiking episodes have been claimed to represent partial upper airway obstruction during sleep, but the mechanism of their appearance is not known...
متن کاملA New Family of Membrane Electron Transporters and Its Substrates, Including a New Cell Envelope Peroxiredoxin, Reveal a Broadened Reductive Capacity of the Oxidative Bacterial Cell Envelope
UNLABELLED The Escherichia coli membrane protein DsbD functions as an electron hub that dispatches electrons received from the cytoplasmic thioredoxin system to periplasmic oxidoreductases involved in protein disulfide isomerization, cytochrome c biogenesis, and sulfenic acid reduction. Here, we describe a new class of DsbD proteins, named ScsB, whose members are found in proteobacteria and Chl...
متن کاملThe validity of the static charge sensitive bed in detecting obstructive sleep apnoeas.
The demand for polysomnographic recordings associated with respiratory control exceeds the capacity of the few existing sleep disorder centres and therefore a simple and inexpensive method is needed for screening and diagnosing sleep-related breathing disorders. The static charge sensitive bed (SCSB) permits long-term recordings of body movements, respiratory movements and the ballistocardiogra...
متن کاملThe 'other' bone sarcomas: prognostic factors and outcomes of spindle cell sarcomas of bone.
We aimed to identify the incidence, outcome and prognostic factors associated with spindle cell sarcomas of bone (SCSB). We studied 196 patients with a primary non-metastatic tumour treated with the intent to cure. The results were compared with those of osteosarcoma patients treated at our hospital during the same period. The overall incidence of SCSB was 7.8% of all patients with a primary bo...
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